Berciaud Histidine
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SelfAssembly of Vesicular Systems
Giant unilamellar vesicles GUVs with sizes ranging from 5 to 100 im are intensely studied in diverse areas, focusing on membrane functions such as adhesion, fusion, fission Luisi and Walde 1999 , or generation and properties of nanotubes and network assemblies. A number of methods to create giant vesicles have been reported. One of the methods is based on a dehydration-rehydration procedure described by Criado and Keller 1987 . As a variant, the rehydration of thin dried films obtained after...
SingleMolecule Microscopy and Force Spectroscopy of Membrane Proteins
Andreas Engel , Harald Janovjak, Dimtrios Fotiadis, Alexej Kedrov, David Cisneros, and Daniel J. M ller 280 11.2 High-Resolution Imaging Using Atomic Force 282 11.2.3 Optimizing Conditions for Contact Mode 283 11.2.4 Determining Oligomeric State and 286 11.2.6 AFM, a Structural Biology Method Complementing 11.2.7 Observing the Diffusion of Single Membrane Proteins 288 11.2.8 Observing Structural Flexibility of Membrane 289 11.2.9 Imaging Conformational Changes of Native Membrane Proteins 292...
Mass Transport in NanotubeVesicle Networks
In order to take full advantage of lipid nanotube-vesicle networks NVNs as systems for performing operations with single molecules, including initiating and controlling chemical reactions, ways to transport materials through the nanotubes are of central importance. There are three fundamental transport mechanisms available Figure 9.3 . The first, Marangoni transport, is based on membrane tension gradients and utilizes the dynamic and fluid character of the bilayer membrane. The second is...
TensionControlled Marangoni Lipid Flow and Intratubular Liquid Flow in Nanotubes
If there is a tension difference along the interface of a fluid system, this can give rise to a flow Bloom et al. 1991 Dommersnes et al. 2005 Finkelstein 1987 . Interfacial tension-driven flows are generally called Marangoni flows and examples of this flow behavior are spreading of a film on an air-water interface or wetting of a dry solid substrate by a film de Gennes 1985 Probstein 1994 . For a film of thickness e, a shear flow in the direction of the tension gradient is given by where z is...
Statistical Analysis
The relevant questions to be answered with SPT are the following. Is the motion of molecules in a 2D biological membrane Brownian, anomalous or confined What are the characteristic parameters of the particular mode of motion, for example, the diffusion coefficient, the degree of anomality, or the confinement size Are these properties invariant or do they depend on the state of the molecule or the environment such as the state of development of the cell Is the ensemble of molecules homogeneous...